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Image Search Results
Journal: Wellcome Open Research
Article Title: A nonlinear and time-dependent leak current in the presence of calcium fluoride patch-clamp seal enhancer
doi: 10.12688/wellcomeopenres.15968.1
Figure Lengend Snippet: On the left shows the time series recordings under the staircase protocol (top panel) ( Lei et al. , 2019a ); right shows the current-voltage (I-V) relationships (at steady state). Experimental recordings are shown in blue, and linear leak estimations are shown in dashed orange. Typical recordings of the leftover current measured on hERG1a transfected CHO cells after applying an I Kr-specific blocker (0.5 µM of E-4031) are shown, where experiments were performed at ( A ) F. Hoffmann-La Roche, Basel ( Lei et al. , 2019a ; Lei et al. , 2019b ) and ( B ) an independent reproduction at Victor Chang Cardiac Research Institute in Sydney. ( C ) Shows a typical recording for an empty well-plate (no biological cells in the solution) in the automated platform.
Article Snippet: In we show measurements taken at: (A) F. Hoffmann-La Roche,
Techniques: Transfection
Journal: Wellcome Open Research
Article Title: A nonlinear and time-dependent leak current in the presence of calcium fluoride patch-clamp seal enhancer
doi: 10.12688/wellcomeopenres.15968.1
Figure Lengend Snippet: On the left shows the time series recordings under the staircase protocol (top panel) from Lei et al. (2019a) ; right shows the current-voltage relationships (fitted with a single exponential function, approximating the steady state relation). Experimental recordings are shown in blue, and linear leak estimations are shown in dashed orange. ( I )–( III ) show the results of Measurements I–III in . ( I ) with internal and external solutions as per automated experiments (similar to those in ); ( II ) with internal and external solutions swapped, the nonlinear portions are now at negative rather than positive voltages; ( III ) same conditions as ( II ) after F − was washed out of the bath — the current becomes much closer to the expected linear leak given by .
Article Snippet: In we show measurements taken at: (A) F. Hoffmann-La Roche,
Techniques: